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KMID : 0950020060320020067
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Journal of Health Science & Medical Technology
2006 Volume.32 No. 2 p.67 ~ p.72
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High-Resolution Melting Assay for the Detection of fliC variations in Salmonella serotypes Dublin and Naestved
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Yoo Young-Jae
Kwon Eun-Hae
Kim Sul-Ki
Kim Hee-Young
Lee Hyun-Jeong
Choi Jeong-Hoon
Choi Hyun-Ju
Hwang Young-Jin
Lim Young-Hee
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Abstract
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High resolution melt curve analysis(HRM) is a simple post-PCR technique which can be used for mutation scanning and genotyping. We identified Salmonella enterica Dublin and S. enterica Naestved which have single base difference between them in fliC gene by HRM. The 102-base pair amplicons expressed different melting temperatures. Using the method, we can identify serotypes even which have a base variant without post-PCR handling, resulting in short analysis time.
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KEYWORD
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High resolution melt curve analysis, genotyping, Salmonella
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